primer n. 1.初級讀本,初學(xué)書;初階,入門(書);(尤指宗教改革前的)小禱告書。 2.【印刷】10點或18點活字的舊稱。 great primer 18 點鉛字。 long primer 10 點鉛字。 primer2 n. 1.裝填火藥者;裝雷管者;起動油泵者。 2.【機械工程】初給器;發(fā)火極;發(fā)火藥;導(dǎo)火線;爆管,雷管;火帽;底火。 3.底漆,首涂油。 4.【生物化學(xué)】引體。
Among 70 arbitrary primers , 13 primers could get enough amplified bands for all strains 這13個引物對11株供試菌株均能擴增出清晰的譜帶。
The procedure of ddrt - pcr applicable for silver staining was optimized by adjusting the amount of several critical reagents , including total rna , anchor primer , arbitrary primer , cdna and dntp 通過調(diào)整ddrt - pcr中總rna 、錨定引物、隨機引物、 cdna和dntp等關(guān)鍵試劑的用量,優(yōu)化了適用于銀染檢測的ddrt - pcr方法。
Among 54 arbitrary primers tested , only 9 primers could generate clear and reproducible fragments in 32 species belonging to 12 genera of the family gryllidae ; 9 primers in 15 species belonging to 7 genera of the family trigonidiidae ; 12 primers in 3 species belonging to the genus oecanthus of the family oecanthidae ; 12 primers in 4 species belonging to 3 genera of the family eneopteridae ; 13 primers in 1 species belonging to the genus homoeogryllus of the family phalangopsidae ; 13 primers in 1 species belonging to the genus sclerogryllus of the family sclerogryllidae ; and 13 primers in 1 species belonging to the genus eulandrevus of the family gryllomorphidae 本研究經(jīng)用54種隨機引物對57種蟋蟀的rapd擴增分析得出以下結(jié)論: 1 )采用rapd技術(shù),首次較系統(tǒng)地對中國蟋蟀總科7科26屬共57種蟋蟀進行了系統(tǒng)學(xué)研究。在所試用的54種隨機引物中篩選出可擴增出清晰可辨且穩(wěn)定的rapd圖譜的引物分別為:蟋蟀科9種、嶺蟋科9種、樹蟋科12種、結(jié)蟋科12種、蛛蟋科13種、鐵蟋科13種、貌蟋科13種。
Rapd ( random amplified polymorphic dna ) , which bases on the polymerase chain reaction ( pcr ) , is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms . the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna , and a small number of fragments will be amplified when the primer anneals on each strand over a length range . if sequence variation is present at the priming site , then a fragment may not be amplied , so the dna polymorphic can be detected Rapd (隨機擴增多態(tài)性dna )技術(shù)是二十世紀90年代發(fā)展起來的一項dna分子多態(tài)性檢測技術(shù),它建立于聚合酶鏈式反應(yīng)( pcr )技術(shù)基礎(chǔ)之上,利用隨機合成的寡聚核苷酸序列為引物(一般為10個bp ) ,分別與dna的兩條單鏈結(jié)合,在dna聚合酶的作用下,對基因組的特定區(qū)域進行pcr擴增,其電泳結(jié)果為不同大小和數(shù)目的dna譜帶即rapd圖譜,可反映基因組相應(yīng)區(qū)域的dna多態(tài)性。
Forty arbitrary primers were used in the analysis of the seven samples , within which five primers produced relatively stable diversity . in total 49 loci were detected by these five primers , including 25 diverse loci ( 51 % ) . pairwise genetic distances were calculated and accordingly cluster analysis was carried out and the genetic dendrogram of the seven samples were generated 在rapd分析中使用了40個隨機引物對7個樣本進行分析,經(jīng)重復(fù)實驗,篩選出了5個多態(tài)性較為穩(wěn)定的引物,測到49個位點,其中25個( 51 )有多態(tài)性。